Caldo brila. Portuguese term or phrase: The coincidence of these results was These methods are laborious and the results for total coliforms are obtained only after 48h incubation, while a further period of 48h incubation is needed to check for the presence of faecal calo 8. View Ideas submitted by the community. The correlation between the two methods was 0. You have native languages that can be verified You can request verification for native languages by completing a simple application that takes only a couple of minutes. View forum View forum without registering on UserVoice.

Author:Mazudal Malatilar
Language:English (Spanish)
Published (Last):5 February 2013
PDF File Size:13.18 Mb
ePub File Size:14.50 Mb
Price:Free* [*Free Regsitration Required]

It is based on reactions of specific microbial enzymes or indicator nutrients of the medium. There was a significant correlation between the results obtained by the two methods for total coliforms r: 0. In addition, results were available as early as 24h.

The group of total coliforms is the main indicator of the microbiological quality of water and food 7 and their presence indicates improper treatment or post-disinfecting contamination. Its use for water analysis was suggested in 3.

In the milk industry, coliform counts are used to indicate hygiene in milk production and post-pasteurization contamination 9. These methods are laborious and the results for total coliforms are obtained only after 48h incubation, while a further period of 48h incubation is needed to check for the presence of faecal coliforms 8.

These time periods are incompatible with the perishable nature of milk. This system is based on enzymes that are not part of the microorganisms. The culture medium offers two active combined substrates, 5-Bromo-4ChloroIndolyl b-d-galactopyranoside X-GAL and the 4-methylumbelliferyl-b-d-glucuronide MUG , which simultaneously detect bacteria from the coliform and E.

The total coliforms produce the b-galactosidase enzyme which cleaves the X-GAL releasing the indicator that produces a change of color in the medium from yellow to greenish blue. For plating, serial decimal dilutions , and of each sample were prepared in 0. Standard methodology for total and faecal coliform enumeration Total coliforms. The multiple tube technique, using 3 dilutions in triplicates, was used following Brasil - MARA 1 , with readings after 24h and 48h of incubation.

The tubes with blurred medium and gas formation in the Durhan tubes were considered positive. Faecal Coliforms. Samples that fermented lactose in the EC broth gas formation in the Durhan tubes and produced indol in the Tryptone broth were considered positive. The dehydrated culture medium was dissolved in mL of bi-distilled sterilized water and distributed in sterile test tubes 9. Escherichia coli The tubes that were positive for total coliforms were observed for fluorescence at UV light nm and for production of indol after addition of Kovacs reagent Laborclin, Curitiba, PR, Brazil.

Positivity for both tests indicated a positive result for E. The MPN of E. Statistical analysis The MPN results were converted to log 10 and submitted to statistical evaluation using Excell 97 and Statistica. The Kappa test 6 and a probabilistic model 2,5 were used. The exact concentration of added E. Among 66 samples with counts for total coliforms, in 37 The correlation r between the methods was 0.

Seven 5. Other seven 5. The coincidence of these results was Other methods, using the same substrates, were evaluated by Venkateswaran et al. In 11 samples 8. The correlation between the two methods was 0. Due to the low number of samples that were positive for faecal coliforms and E. Any alteration to greenish blue or similar tones indicated a positive result.

The opacity of the milk did not interfere in color production or fluorescence. Results are easily visualized, and the time required for MPN results for both groups of microorganisms is only 24h. No false positive or false negative results were observed. Cohen, J. A coefficient of agreement for nominal scale.

Education and Psychological Measurement , , Franco, B. Gale, P. Evaluation of a rapid, defined substrate technology method for enumeration of total coliforms and Escherichia coli in chlorinated drinking water. Microbiol , , Gart, J. Comparison of a screening test and a reference test in epidemiologic studies.

Landis, J. The measurement of observer agreement for categorical data. Biometrics , , Matner, R. Food Prot. Nelson, C. Senik, G. Comparison of dry culture medium and conventional plating techniques for enumeration of bacteria in pasteurized fluid milk. Dairy Sci. Venkateswaran, K. Comparison of comercialy Escherichia coli in foods.

Services on Demand Journal. All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License. How to cite this article.



The dish is a meat stew , traditionally made from beef but occasionally made from goat meat or mutton. The dish is often served at celebratory occasions, such as weddings and baptisms, and holidays, such as Christmas and Easter. Preparation techniques vary, but the dish is often served with corn tortillas , onion , cilantro and lime. The meat is marinated in an adobo made of vinegar, dried chiles, herbs, and spices before being cooked in a broth. Restaurants or street carts that serve birria are known as birrierias and exist throughout Mexico, especially in Jalisco and its capital, Guadalajara. However, neighboring Mexican states have their own variations of the dish: the birria estilo Zacatecas of Zacatecas and the birria de Colima of Colima.








Related Articles